A simple, straightforward method for modeling a STED microscope using Zemax geometrical optics ray tracing principles, while still achieving a realistic spot size
Stimulated emission depletion (STED) microscopy is a powerful tool for the study of sub-micron samples, especially in biology. In our published article in MicroscopyToday magazine, we present a simple, straightforward method for modeling a STED microscope using Zemax geometrical optics ray tracing principles, while still achieving a realistic spot size, with an output behavior consistent with the real, diffractive behavior of a STED de-excitation spot. This method can simplify the design of custom STED setups by proper modeling of the resolution performance.
